UHPLC-MS Method for the Analysis of the Molecular Adjuvant Sulfavant A

A fast and sensitive method that is based on Ultra High Performance Liquid Chromatography coupled with Resolution Mass Spectrometry (UHPLC-HRMS) for the measurement of Sulfavant A, a molecular adjuvant sulfolipid skeleton, described. The has been validated over linearity range 2.5–2000 ngmL−1 using deuterated derivative (d70-Sulfavant A) as internal standard. Chromatographic separation UHPLC Kinetex® 2.6 µm PS C18 column gradient methanol in 0.32 mM ammonium hydroxide solution buffered at pH 8. lowest limit quantification was 6.5 ngmL−1. analytical procedure tested an extract mice lung spiked 30, 300, 1500 ng A. analysis revealed precision accuracy value (as mean all quality control samples analyzed) 4.7% 96% MeOH 6.4% 93.4% extracts, respectively.